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1School of Public Health,Nanjing Medical University, 2School of Pharmacy,Nanjing Medical University, 3State Key Laboratory of Materials-Oriented Chemical Engineering, College of Chemistry and Chemical Engineering,Nanjing Tech University, 4Department of Medical Oncology, Jiangsu Cancer Hospital, Jiangsu Institute of Cancer Research,The Affiliated Cancer Hospital of Nanjing Medical University
Chapters
- 00:04Title
- 00:52Proliferation/Cytotoxicity Experiment and Data Acquisition
- 03:54Case 1: Regeneration of Electronic Plates after Cytotoxicity Evaluation of Anti-cancer Drug
- 05:53Case 2: Regeneration of Electronic Plates after Drug Delivery using Mesoporous Material
- 07:34Assessment of Regeneration Effect
- 10:22Results: Evaluationof Regenerated Electronic Plates
- 12:01Conclusion
Este protocolo descreve uma estratégia geral para regenerar microeletrodos de ouro matriz comerciais equipados para um analisador de célula livre de rótulo visto economia sobre as alta execução custos ofmicrochip ensaios baseados. O processo de regeneração inclui digestão do trypsin, enxaguando com água, etanol e uma etapa de fiação, que permite o uso repetido de microchips.
