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Genetics
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JoVE Journal Genetics
Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing
 

Microinjection of CRISPR/Cas9 Protein into Channel Catfish, Ictalurus punctatus, Embryos for Gene Editing

Article DOI: 10.3791/56275-v • 16:08 min • January 20th, 2018
January 20th, 2018 •
*1,2, *1,3, 1, 4, 4, 5, 5, 1
1School of Fisheries, Aquaculture and Aquatic Sciences, Auburn University, 2Department of Animal Wealth Development, Faculty of Veterinary Medicine, Suez Canal University, 3Anatomy and Embryology Department, Faculty of Veterinary Medicine, Cairo University, 4Department of Molecular Physiology and Biophysics, Vanderbilt University, 5Life Science Institute, University of Michigan
* These authors contributed equally

챕터

요약
Automatic Translation

A simple and efficient microinjection protocol for gene editing in channel catfish embryos using the CRISPR/Cas9 system is presented. In this protocol, guide RNAs and Cas9 protein were microinjected into the yolk of one-cell embryos. This protocol has been validated by knocking out two channel catfish immune-related genes.

Tags

Keywords: CRISPR/Cas9 Microinjection Channel Catfish Ictalurus Punctatus Gene Editing TICAM1 RBL Broodstock Selection Spawning LHRHA Hormone Injection Embryo Protocol Functional Genomics
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