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Chapters
- 00:05Title
- 00:54Purification of Naïve Human CD4+ Cells, Polarization to Th1 and TH2 Cells, and Nuclei Extraction
- 03:18Transposition Reaction and Library Amplification
- 04:33Size Selection of ATAC-seq Libraries
- 06:48Results: Quality Control and Next Generation Sequencing Reads
- 08:07Conclusion
Assay for Transposase-Accessible Chromatin coupled with high-throughput sequencing (ATAC-seq) is a genome-wide method to uncover accessible chromatin. This is a step-by-step ATAC-seq protocol, from molecular to the final computational analysis, optimized for human lymphocytes (Th1/Th2). This protocol can be adopted by researchers without prior experience in next-generation sequencing methods.
