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JoVE Journal Cancer Research
Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment
 

Conditional Knockdown of Gene Expression in Cancer Cell Lines to Study the Recruitment of Monocytes/Macrophages to the Tumor Microenvironment

Article DOI: 10.3791/56333-v • 10:59 min • November 23rd, 2017
November 23rd, 2017 •
1,2, 1,2,3
1Division of Hematology, Oncology and Blood and Marrow Transplantation, Department of Pediatrics, University of Southern California, 2The Saban Research Institute of Children's Hospital Los Angeles, 3Department of Biochemistry and Molecular Medicine, University of Southern California

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요약
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This protocol serves as a scheme for setting up a functional Tet-ON system in cancer cell lines and its subsequent use, in particular for studying the role of tumor cell-derived proteins in recruitment of monocytes/macrophages to the tumor microenvironment.

Tags

Conditional Knockdown Gene Expression Cancer Cell Lines Recruitment Monocytes Macrophages Tumor Microenvironment In Vitro Cross-talk Immune System Single Vector Cloning Multiple Clones Low Levels Of Thickness Purification Human Monocytes Viral Particles Transfect HEK-293 Cells PLKO-tet-on ShRNA PsPAX Packaging Plasmid PMD2.G Envelope-expressing Plasmid Lentiviral Production Sodium Butyrate HEPES
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