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测定急性髓系白血病可测残留病和白血病干细胞的骨髓标本和过程综合协议
JoVE 신문
암 연구학
This content is Free Access.
JoVE 신문 암 연구학
Comprehensive Protocol to Sample and Process Bone Marrow for Measuring Measurable Residual Disease and Leukemic Stem Cells in Acute Myeloid Leukemia
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测定急性髓系白血病可测残留病和白血病干细胞的骨髓标本和过程综合协议

DOI:
10.3791/56386-v

•

09:57 min

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March 05, 2018

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, , , , , , , , , , , , , , , , ,
1Department of Hematology,VU University Medical Center, 2Pediatric Oncology/Hematology,VU University Medical Center, 3Janssen Research & Development, LLC, 4Princess Máxima Center for Pediatric Oncology

Chapters

  • 00:05Title
  • 01:06Bone Marrow Aspiration and Smear Preparation
  • 03:16Flow Cytometry Setup
  • 05:53Cell Staining for Flow Cytometry
  • 07:45Results: Representative Leukemia-associated Immunophenotype (LAIP) Gating
  • 08:46Conclusion

Summary

자동 번역

자동 번역

检测微量或可测量残留疾病 (MRD) 是一种重要的预后标志物, 用于提炼风险评估和预测急性髓细胞白血病 (AML) 复发。这些全面的指导方针和建议采用最佳做法, 以一致和准确地识别和检测 MRD, 有助于作出有效的反洗钱治疗决定。

Tags

Bone MarrowAcute Myeloid LeukemiaMeasurable Residual DiseaseLeukemia Stem CellsSamplingProcessingProtocolHematologyDiagnosisTreatment Monitoring

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