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Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation
 

Assessment of Dictyostelium discoideum Response to Acute Mechanical Stimulation

Article DOI: 10.3791/56411-v 10:40 min November 9th, 2017
November 9th, 2017

챕터

요약

Here we describe methods for assessing cellular response to acute mechanical stimulation. In the microscopy-based assay, we examine localization of fluorescently-labeled biosensors following brief stimulation with shear flow. We also test activation of various proteins of interest in response to acute mechanical stimulation biochemically.

Tags

Keywords: Dictyostelium Discoideum Mechanical Stimulation Intracellular Signal Transduction Cell Migration Chemoattractant Klebsiella Aerogenes Macropinosome SM Plate DB Buffer Aggregation-competent Dictyostelium
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