Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / Modified Roller Tube Method for Precisely…
JoVE Journal
Neuroscience

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE Journal Neuroscience
Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System
 

Modified Roller Tube Method for Precisely Localized and Repetitive Intermittent Imaging During Long-term Culture of Brain Slices in an Enclosed System

Article DOI: 10.3791/56436-v • 09:52 min • December 28th, 2017
December 28th, 2017 •
*1, *1, *1, 1, 1,2, 1, 1,3, 1, 1
1Department of Biochemistry and Molecular Biology and Molecular, Cellular and Integrated Neuroscience Program, Colorado State University, 2IBMC-Instituto de Biologia Molecular e Celular, i3S-Instituto de Investigaçãoe Inovação em Saúde, ICBAS, Universidade do Porto, 3Denali Therapeutics
* These authors contributed equally

챕터

요약
Automatic Translation

Presented here is a modified roller tube method for culturing and intermittent high-resolution imaging of rodent brain slices over many weeks with precise repositioning on photoetched coverslips. Neuronal viability and slice morphology are well maintained. Applications of this fully enclosed system using viruses for cell-type specific expression are provided.

Tags

Keywords: Brain Slice Culture Long-term Culture Repetitive Imaging Fluorescence Imaging Enclosed System Roller Tube Method Photo Etched Coverslips Viral Vectors Neurological Disorders Synapse Alteration Rodent Models
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter