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Developmental Biology

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JoVE Journal Developmental Biology
Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs)
 

Rapid Detection of Neurodevelopmental Phenotypes in Human Neural Precursor Cells (NPCs)

Article DOI: 10.3791/56628-v • 10:47 min • March 2nd, 2018
March 2nd, 2018 •
*1, *1, 1, 2, 3, 3, 4, 5, 2, 1
1Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, 2Center for Advanced Biotechnology and Medicine, Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, 3The Child Health Institute of NJ, Department of Obstetrics, Gynecology, and Reproductive Services, Rutgers Robert Wood Johnson Medical School, 4The Child Health Institute of NJ, Department of Neuroscience and Cell Biology, Rutgers Robert Wood Johnson Medical School, 5Department of Genetics, Rutgers University
* These authors contributed equally

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요약
Automatic Translation

Neurodevelopmental processes such as proliferation, migration, and neurite outgrowth are often perturbed in neuropsychiatric diseases. Thus, we present protocols to rapidly and reproducibly assess these neurodevelopmental processes in human iPSC-derived NPCs. These protocols also allow the assessment of the effects of relevant growth factors and therapeutics on NPC development.

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Keywords: Neural Precursor Cells Neurodevelopmental Phenotypes Neural Development Cell Characterization Cell Culture Cell Detachment Cell Harvesting Tritiated Thymidine Cell Proliferation Neurodevelopmental Disorders Neuropsychiatric Disorders Rapid And Reproducible Method
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