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Chapters
- 00:05Title
- 00:42Coating of Microtiter Plates with α4β1 Integrin for Ex Utero Allantois Explant Culture
- 01:54Uterus Dissection from a Pregnent Mouse at DPC 8
- 03:24Embryo and Allantois Dissection and Ex Utero Allantois Cultre
- 06:18Staining Explanted Allantoides with Endothelial Cell Marker CD31
- 08:07Results: Allantois Attachment and Immunohistochemistry
- 08:54Conclusion
We describe an in vitro assay to model chorioallantoic attachment, the first step in placenta formation. The protocol demonstrates the dissection and explant culture of murine allantoides on immobilized α4β1 integrin. Allantois attachment is evaluated microscopically at pre-determined time points.
