Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / Using CRISPR/Cas9 Gene Editing to Investigate the …
JoVE Journal
Cancer Research

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE Journal Cancer Research
Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells
 

Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells

Article DOI: 10.3791/56726-v • 10:21 min • January 5th, 2018
January 5th, 2018 •
1, 1,2,3
1Division of Hematology, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, 2Broad Institute, 3Dana-Farber Cancer Institute, Harvard Medical School

챕터

요약
Automatic Translation

Targeted gene editing using CRISPR/Cas9 has greatly facilitated the understanding of the biological functions of genes. Here, we utilize the CRISPR/Cas9 methodology to model calreticulin mutations in cytokine-dependent hematopoietic cells in order to study their oncogenic activity.

Tags

Keywords: CRISPR/Cas9 Gene Editing Calreticulin Oncogenic Activity Cytokine Dependent Hematopoietic Cells Hematological Malignancies LentiGuide-Puro Vector BsmB1 Restriction Enzyme Phosphatase Ligation STBL3 Cells HEK293T Cells Transfection
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter