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动态和电泳光散射的 DNA-磁性颗粒结合分析

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화학

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DNA-magnetic Particle Binding Analysis by Dynamic and Electrophoretic Light Scattering

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动态和电泳光散射的 DNA-磁性颗粒结合分析

DOI:

10.3791/56815-v

•

10:35 min

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November 09, 2017

•

Yazan Haddad², Simona Dostalova², Jiri Kudr², Ondrej Zitka², Zbynek Heger², Vojtech Adam²

¹Department of Chemistry and Biochemistry,Mendel University in Brno, ²Central European Institute of Technology,Brno University of Technology

Chapters

00:05Title
00:49Synthesis of Magnetic Nanoparticles (MNPs)
03:07Chemical Modification of Magnetic Nanoparticles
05:53Magnetic Particle Size Analysis Using DLS
06:46Testing Efficiency of DNA Isolation Using Quantitative PCR
07:58Magnetic Particle Zeta Potential Analysis Using DLS
08:37Results: Zeta Potential of Representative Magnetic Particles Over Time in Presence and Absence of DNA
09:57Conclusion

Summary

자동 번역

该协议描述了磁性粒子的合成, 并通过动态和电泳光散射对其 DNA 结合特性进行了评价。该方法着重于监测粒子大小的变化, 它们的多和粒子表面的泽塔电位, 它们在 DNA 等材料的结合中起着重要作用。

Tags

Keywords: DNA-magnetic Particle Binding Dynamic Light Scattering (DLS)Electrophoretic Light Scattering (ELS)Particle Size Polydispersity Zeta Potential DNA Isolation Magnetic Particles Biotechnology Molecular Biology Polyethylenimine Tetraethyl Orthosilicate (3-aminopropyl)triethoxysilane Tris-HCl Buffer

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