Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

Krista M. Armbruster; Timothy C. Meredith

doi:10.3791/56842

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Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

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Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

DOI:

10.3791/56842-v

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10:59 min

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May 21, 2018

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Krista M. Armbruster, Timothy C. Meredith

¹Department of Biochemistry and Molecular Biology,Pennsylvania State University

Chapters

00:04Title
00:47Cell Growth and Lysis
02:23Enrichment of Lipoproteins by TX-114 Phase Partitioning
05:05SDS-PAGE, Electroblotting, and Staining with Ponceau S
06:30Tryptic Digestion, Lipopeptide Extraction from Nitrocellulose Membrane, Deposition onto MALDI Target, and Data Acquisition
08:47Results: MALDI-TOF MS Analysis of N-terminal Lipopeptides
10:17Conclusion

Summary

자동 번역

The enrichment of bacterial lipoproteins using a non-ionic surfactant phase partitioning method is described for direct use in TLR assays or other applications. Further steps are detailed to prepare N-terminal tryptic lipopeptides for structural characterization by mass spectrometry.

Enrichment of Bacterial Lipoproteins and Preparation of N-terminal Lipopeptides for Structural Determination by Mass Spectrometry

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