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Chapters
- 00:05Title
- 01:25Preparation of Cultured Cancer Cells
- 02:35Preparation of Mouse and Surgery
- 06:06Sciatic Nerve Extraction
- 07:24Nerve Processing and Quantification
- 08:26Results: H&E Longitudinal Sections of Sciatic Nerves Injected with Panc02H7 Cancer Cells in Wild Type and NCAM KO Mice
- 09:10Conclusion
We describe an in vivo murine model of perineural invasion by injecting syngeneic pancreatic cancer cells into the sciatic nerve. The model allows for quantification of the extent of nerve invasion, and supports investigation of the cellular and molecular mechanisms of perineural invasion.
