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流式细胞仪估测原发性急性髓系白血病和患者源性移植中白血病干细胞的诊断和随访

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Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

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流式细胞仪估测原发性急性髓系白血病和患者源性移植中白血病干细胞的诊断和随访

DOI:

10.3791/56976-v

•

09:01 min

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March 26, 2018

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Thomas Boyer², Fanny Gonzales³, Adriana Plesa, Pauline Peyrouze, Adeline Barthelemy, Soizic Guihard, Bruno Quesnel⁵, Christophe Roumier², Claude Preudhomme², Meyling Cheok

¹Laboratoire d’hématologie,CHU Lille, ²Univ. Lille, Inserm, CHU Lille, UMR-S 1172 – JPArc – Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer, ³Service d’hématologie pédiatrique, hôpital Jeanne de Flandre,CHU Lille, ⁴Laboratoire d’hématologie CH Lyon Sud, ⁵Service des Maladies du Sang, hôpital Claude Huriez,CHU Lille

Chapters

00:04Title
00:58Sample Preparation and Lysis of Red Blood Cells
02:26Patient Derived Xenografts and Staining
04:50Flow Cytometry and Gating Strategy
06:14Results: Parametric Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia
07:57Conclusion

Summary

자동 번역

通过流式细胞术, 我们勾勒出一种检测白血病干细胞标记物同时表达的协议。我们展示了如何量化三个祖群体和一个假定的多项种群的成熟程度。我们证实了这些人群存在于相应的病人衍生的移植。

Tags

Flow Cytometry Leukemia Stem Cells Acute Myeloid Leukemia Patient-derived Xenografts Diagnosis Follow-up Mononuclear Cells Density Gradient Red Blood Cell Lysis NSG Mouse Xenograft Submandibular Blood Collection

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