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Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy
 

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy

Article DOI: 10.3791/57164-v • 06:51 min • August 2nd, 2018
August 2nd, 2018 •
1,2, 3, 4, 1,2,5,6,7
1Tumour Targeting Laboratory, Olivia Newton-John Cancer Research Institute, 2School of Cancer Medicine, La Trobe University, 3Centre for Micro-Photonics, Faculty of Science, Engineering and Technology, Swinburne University of Technology, 4LIMS Bioimaging Facility, La Trobe Institute for Molecular Science, La Trobe University, 5Department of Medical Oncology, Olivia Newton-John Cancer and Wellnes Centre, Austin Health, 6Department of Medicine, University of Melbourne, 7Department of Molecular Imaging and Therapy, Austin Health

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요약
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Antibodies that bind to target receptors on the cell surface can confer conformation and clustering alterations. These dynamic changes have implications for characterizing drug development in target cells. This protocol utilizes confocal microscopy and image correlation spectroscopy through ImageJ/FIJI to quantify the extent of receptor clustering on the cell surface.

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Keywords: Confocal Microscopy Cell Surface Receptor Aggregation Image Correlation Spectroscopy EGF Receptor Immunocytochemistry Image Processing FD Math Image Correlation
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