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JoVE Journal
Immunology and Infection
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Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis
 

Collection and Processing of Lymph Nodes from Large Animals for RNA Analysis: Preparing for Lymph Node Transcriptomic Studies of Large Animal Species

Article DOI: 10.3791/57195-v 12:54 min May 19th, 2018
May 19th, 2018

챕터

요약

This protocol provides an overview of procedures for the isolation of RNA for the transcriptomic profiling of lymph node tissues from large animals, including steps in the identification and excision of lymph nodes from livestock and wildlife, sampling approaches to provide consistency across multiple animals, and considerations plus representative results for the post-collection preservation and processing for RNA analysis.

Tags

Keywords: Lymph Nodes RNA Analysis Large Animals Transcriptomic Studies Zoonotic Diseases Brucellosis E. Coli O157:H7 Deer Elk Tissue Harvesting Tissue Collection RNA Preservation RNAlater Necropsy Veterinary Research Molecular Biology
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