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Journal / Genetics / Manipulation of Ploidy in Caenorhabditis elegans
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Genetics

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JoVE Journal Genetics
Manipulation of Ploidy in Caenorhabditis elegans
 

Manipulation of Ploidy in Caenorhabditis elegans

Article DOI: 10.3791/57296-v • 07:54 min • March 15th, 2018
March 15th, 2018 •
1, 1,2, 1, 1,3, 1,2,3
1Brooklyn College, Biology Department, City University of New York, 2The Graduate Center, Biology Department, City University of New York, 3Advanced Science Research Center, City University of New York

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요약
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This method allows for the generation of tetraploid and triploid Caenorhabditis nematodes from any diploid strain. Polyploid strains generated by this method have been used to study chromosome interactions in meiotic prophase, and this method is useful for examining important basic questions in cell, developmental, evolutionary, and cancer biology.

Tags

Ploidy Manipulation Tetraploid Strains Diploids Genotype Karyotype Whole Genome Polyploidization Gene Dosage Biological Scaling Extracellular Signaling Genome Instability Drug Resistance Adaptation To Stress Mechanisms Of Speciation Efficient Technique Versatile Technique Simple Technique Multicellular Organisms Stable Tetraploids Rec-8 Mutants Chromosome Segregation Phenotype Diploid Sperm And Oocytes Double-stranded RNA Expression Escherichia Coli NGM Agar Plates
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