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重组胶原蛋白 i. 肽载体在生物反应器中的应用及其作为细胞传递系统的潜力
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Recombinant Collagen I Peptide Microcarriers for Cell Expansion and Their Potential Use As Cell Delivery System in a Bioreactor Model
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重组胶原蛋白 i. 肽载体在生物反应器中的应用及其作为细胞传递系统的潜力

DOI:
10.3791/57363-v

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08:43 min

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February 07, 2018

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, , , ,
1Department Tissue Engineering and Regenerative Medicine,University Hospital Wuerzburg, 2Translational Center Regenerative Therapies (TLC-RT),Fraunhofer Institute for Silicate Research ISC, 3Fujifilm Manufacturing Europe B.V.

Chapters

  • 00:05Title
  • 00:55Set-up and Sterilization of Spinner Flasks, Recombinant Collagen I Peptide (RCP) Microcarriers, and Bioreactor
  • 02:09Culture of hBMSCs and HDMECs on RCP Macroporous Microcarriers
  • 03:29Biomatrix Seeding and Start of Bioreactor System for HDMECs
  • 05:05Addition of RFP-HDMECs to the Biomatrix
  • 06:16Results: Cell Expansion on Macroporous Microcarriers and Cell Delivery to a Biomatrix in a Bioreactor Model
  • 07:19Conclusion

Summary

자동 번역

자동 번역

提出了大孔载体的细胞扩展协议及其在灌注生物反应器中的传递系统, 以细胞组织基质为种子。我们还包括不同的技术, 以确定细胞增殖和细胞在载体培养的生存能力。此外, 我们还展示了生物反应器培养后细胞的功能。

Tags

Recombinant Collagen IPeptide MicrocarriersCell ExpansionCell DeliveryBioreactorVascularizationSpinner FlaskMacroporous MicrocarriersDulbecco’s Phosphate Buffered SalineAutoclaveCulture MediumHuman Bone Marrow Derived Cells

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