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Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology
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Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology

Identification of Dopamine D1-Alpha Receptor Within Rodent Nucleus Accumbens by an Innovative RNA In Situ Detection Technology

DOI:
10.3791/57444-v

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07:25 min

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March 27, 2018

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, , , ,
1Program in Behavioral Neuroscience, Department of Psychology,University of South Carolina

Chapters

  • 00:04Title
  • 01:00Preparation of Fresh Frozen Brain Sections
  • 02:35RNA In Situ Hybridization Fluorescent Multiplex Assay
  • 05:12Results: Drd1α RNA Level in Nucleus Accumbens Region of F344/N Rats
  • 06:35Conclusion

Summary

자동 번역

자동 번역

Identification of dopamine D1-alpha receptor in the nucleus accumbens is critical for clarifying D1 receptor dysfunction during a central nervous system disease. We performed a novel RNA in situ hybridization assay to visualize single RNA molecules in a specific brain area.

Tags

RNA In-situ HybridizationDopamine D1-alpha ReceptorNucleus AccumbensCentral Nervous System DiseaseDopaminergic DysfunctionTarget MRNABrain SectioningPre-treatmentMultiplex Assay

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