Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Biochemistry

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast
 

Evaluation of the Impact of Protein Aggregation on Cellular Oxidative Stress in Yeast

Article DOI: 10.3791/57470-v 11:04 min June 23rd, 2018
June 23rd, 2018

챕터

요약

Protein aggregation elicits cellular oxidative stress. This protocol describes a method for monitoring the intracellular states of amyloidogenic proteins and the oxidative stress associated with them, using flow cytometry. The approach is used to study the behavior of soluble and aggregation-prone variants of the amyloid-β peptide.

Tags

Keywords: Protein Aggregation Cellular Oxidative Stress Saccharomyces Cerevisiae Yeast Amyloidogenic Protein A-beta-42 GFP Flow Cytometry Recombinant Protein Expression Galactose Induction
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter