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JoVE Journal Cancer Research
Utilization of Ultrasound Guided Tissue-directed Cellular Implantation for the Establishment of Biologically Relevant Metastatic Tumor Xenografts
 

Utilization of Ultrasound Guided Tissue-directed Cellular Implantation for the Establishment of Biologically Relevant Metastatic Tumor Xenografts

Article DOI: 10.3791/57558-v • 07:50 min • May 25th, 2018
May 25th, 2018 •
1, 1, 1, 2, 3, 4, 2,5, 5, 1
1Departments of Surgery, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School, 2Departments of Pathology, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School, 3Unit for Laboratory Animal Medicine, The University of Michigan Medical School, 4Departments of Radiology, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School, 5Departments of Pediatrics, C.S Mott Children's and Women's Hospital, The University of Michigan Medical School

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요약
Automatic Translation

Here, we present a protocol to utilize ultrasound-guided injection of neuroblastoma (NB) and Ewing's sarcoma (ES) cells (established cell lines and patient-derived tumor cells) at biologically relevant sites to create reliable preclinical models for cancer research.

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Keywords: Ultrasound-guided Tissue-directed Cellular Implantation Metastatic Tumor Xenografts Patient-derived Neuroblastoma Orthotopic Xenograft Cancer Biology Translational Research Tumor Evolution Therapeutic Responses Metastases Preclinical Studies Drug Discovery Tumor Dissociation Cell Suspension Tissue Forceps Tissue Dissociater Cell Strainer Hemocytometer
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