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Neuroscience

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Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ
 

Quantifying Microglia Morphology from Photomicrographs of Immunohistochemistry Prepared Tissue Using ImageJ

Article DOI: 10.3791/57648-v 08:44 min June 5th, 2018
June 5th, 2018

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요약

Microglia are brain immune cells that survey and react to altered brain physiology through morphologic changes which may be evaluated quantitatively. This protocol outlines an ImageJ based analysis protocol to represent microglia morphology as continuous data according to metrics such as cell ramification, complexity, and shape.

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Keywords: Microglia Morphology Immunohistochemistry ImageJ Skeleton Analysis Fractal Analysis Neuroinflammmatory Continuous Variables Iba1 FFT Bandpass Filter Unsharp Mask Despeckle Binary Skeletonize
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