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分离绿色荧光蛋白系统可视化细菌在感染过程中传递的效应

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Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection

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分离绿色荧光蛋白系统可视化细菌在感染过程中传递的效应

DOI:

10.3791/57719-v

•

07:25 min

•

May 24, 2018

•

Hye-Young Lee, So Eui Lee, Jongchan Woo, Doil Choi, Eunsook Park

¹Department of Plant Science, Plant Genomics and Breeding Institute, College of Agriculture and Life Science,Seoul National University, ²Department of Bioindustry and Bioresource Engineering,Sejong University

Chapters

00:04Title
00:49Preparation of Plant Materials and Pseudomonas Culture
02:09Transient Expression of Organelle-targeted sfGFP1-10^OPT in N. benthamiana
03:58Inoculation of Pseudomonas and Observation of sfGFP Signal via Confocal Microscopy
05:46Results: Split Superfolder Fluorescent Protein System: A Useful Tool to Examine the Dynamics of Effectors in Host Plants
06:32Conclusion

Summary

자동 번역

以荧光蛋白为基础的方法来监测细菌分泌到宿主细胞中的效应, 这是很有挑战性的。这是由于荧光蛋白与 III. 型分泌系统之间的不相容。在这里, 一个优化的分裂 superfolder GFP 系统是用来可视化的效应, 细菌分泌的宿主植物细胞。

Tags

Keywords: Split Green Fluorescent Protein Effectors Plant-microbe Interaction Type III Secretion System Pseudomonas Syringae Agrobacterium Tumefaciens Infiltration Visualization

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