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JoVE Journal
Immunology and Infection

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Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes
 

Identification of Virulence Markers of Mycobacterium abscessus for Intracellular Replication in Phagocytes

Article DOI: 10.3791/57766-v 08:34 min September 27th, 2018
September 27th, 2018

챕터

요약

Here, we present two protocols to study Phagocyte-Mycobacterium abscessus interactions: the screening of a transposon mutant library for bacterial intracellular deficiency and the determination of bacterial intracellular transcriptome from RNA sequencing. Both approaches provide insight into the genomic advantages and transcriptomic adaptations enhancing intracellular bacteria fitness.

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Keywords: Mycobacterium Abscessus Virulence Markers Intracellular Replication Phagocytes Mutant Library Screening Transcriptomic Studies Acanthamoeba Castellani Amoeba Co-culture Amikacin Escherichia Coli Cell Lysis Columbia Agar Plates
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