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斑马鱼胰岛单细胞分辨率钙成像对β细胞功能的分析

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Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets

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斑马鱼胰岛单细胞分辨率钙成像对β细胞功能的分析

DOI:

10.3791/57851-v

•

08:50 min

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July 03, 2018

•

Sharan Janjuha², Sumeet Pal Singh, Nikolay Ninov²

¹Center for Molecular and Cellular Bioengineering,TU Dresden, ²Paul Langerhans Institute Dresden of the Helmholtz Center Munich at the University Hospital Carl Gustav Carus of TU Dresden

Chapters

00:04Title
00:33Zebrafish Primary Islet Dissection and Mounting
02:20Ex Vivo Live-imaging of GCaMP Fluorescence Intensity in Zebrafish Primary Islets
05:11Quantification of GCaMP Fluorescence Trace for Individual Beta-cells
07:14Results: Single-cell Calcium Imaging in Zebrafish Islet Beta Cells
08:15Conclusion

Summary

자동 번역

β细胞功能是重要的血糖稳态, 这是评估在单细胞分辨率使用基因编码的记者, 钙流入。

Tags

Beta-cell Calcium Imaging Zebrafish Islets Beta-cell Function Calcium Oscillations Single-cell Resolution Ex Vivo Live Imaging GCaMP Fluorescence Fibrinogen-thrombin Mold MKO2 Fluorescence

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