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抗菌肽定位的细菌 Spheroplasts 和原生质体的生产与可视化

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Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization

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抗菌肽定位的细菌 Spheroplasts 和原生质体的生产与可视化

DOI:

10.3791/57904-v

•

10:13 min

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August 11, 2018

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Dania M. Figueroa, Heidi M. Wade², Katrina P. Montales, Donald E. Elmore², Louise E.O. Darling³

¹Biochemistry Program,Wellesley College, ²Department of Chemistry,Wellesley College, ³Department of Biological Sciences,Wellesley College

Chapters

00:04Title
01:14Gram-negative E. coli Spheroplast Preparation
04:12Gram-positive B. megatherium Protoplast Preparation
05:58<E. coli Spheroplast and B. megatherium Protoplast Visualization and Anticrobial Peptide (AMP) Localization
07:49Results: Representative High-resolution AMP Visualization
09:39Conclusion

Summary

자동 번역

在这里, 我们提出了一个生产革兰氏阴性大肠杆菌 (大肠杆菌) spheroplasts 和革兰氏阳性芽孢杆菌巨大(巨大) 原生质体的协议, 以清楚地形象化和快速表征多肽-细菌的相互作用。这为定义膜定位和转运肽提供了系统的方法。

Tags

Keywords: Bacterial Spheroplasts Bacterial Protoplasts Antimicrobial Peptides Cell Membrane Localization Gram-negative Gram-positive E. Coli B. Megaterium Cephalexin Bacterial Filaments Cell Imaging Membrane-active Agents Cell-penetrating Peptides

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