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综合结构质谱分析蛋白质结构和蛋白质配体配合物
JoVE 신문
화학
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JoVE 신문 화학
Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
DOI:

07:33 min

October 15, 2018

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Chapters

  • 00:04Title
  • 00:45Native MS Acquisition and Analysis for Investigating Protein Complexes and Protein-Ligand Complexes
  • 03:51Acquiring and Analyzing IM-MS
  • 04:34In-Solution Disruption of Protein Complexes for Native MS and IM-MS Led Structure Determination and Investigating Protein Complex Stability using Collision Induced Unfolding
  • 05:45Results: Integrative Structural Mass Spectrometry of Protein Architectures and Protein-Ligand Complexes
  • 07:05Conclusion

Summary

자동 번역

质谱 (MS) 已成为研究大分子组件结构和动力学的重要工具。在这里, 我们整合了基于 MS 的方法来询问蛋白质复合形成和配体结合。

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