Journal
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Análisis de arquitecturas de proteínas y complejos de proteína-ligando por espectrometría de masa estructural integrativo
JoVE 신문
화학
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JoVE 신문 화학
Analyzing Protein Architectures and Protein-Ligand Complexes by Integrative Structural Mass Spectrometry
DOI:

07:33 min

October 15, 2018

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Chapters

  • 00:04Title
  • 00:45Native MS Acquisition and Analysis for Investigating Protein Complexes and Protein-Ligand Complexes
  • 03:51Acquiring and Analyzing IM-MS
  • 04:34In-Solution Disruption of Protein Complexes for Native MS and IM-MS Led Structure Determination and Investigating Protein Complex Stability using Collision Induced Unfolding
  • 05:45Results: Integrative Structural Mass Spectrometry of Protein Architectures and Protein-Ligand Complexes
  • 07:05Conclusion

Summary

자동 번역

Espectrometría de masas (MS) se ha convertido en una herramienta importante para la investigación de la estructura y la dinámica de asambleas macromoleculares. Aquí, integramos los enfoques basados en la MS para interrogar la formación de complejos de proteína y de ligando.

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