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基于交配的酵母超表达文库筛选

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Mating-based Overexpression Library Screening in Yeast

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基于交配的酵母超表达文库筛选

DOI:

10.3791/57978-v

•

11:39 min

•

July 06, 2018

•

Elliott Hayden, Shuzhen Chen, Abagail Chumley, Quan Zhong, Shulin Ju

¹Department of Biological Sciences,Wright State University

Chapters

00:04Title
00:39Yeast Transformation in 96-well Format
05:48Mating Between Cells Containing Library Genes and Query Yeast
08:20Spotting Assay
09:12Results: Successful Screening for Genes that Modify FUS Toxicity
10:44Conclusion

Summary

자동 번역

本文提出了一种基于交配的方法, 利用阵列质粒库, 促进萌芽酵母的超表达筛查。

Tags

Keywords: Mating-based Overexpression Library Screening Yeast Neurodegenerative Disease Genetic Factors Cellular Processes Stress Conditions Yeast Peptone Dextrose Haploid Yeast Lithium Acetate Salmon Sperm DNA Transformation Buffer

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