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Developmental Biology

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JoVE Journal Developmental Biology
Encellede transkriptom analyser af mus i bugspytkirtlen endokrine celler
 
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Encellede transkriptom analyser af mus i bugspytkirtlen endokrine celler

Article DOI: 10.3791/58000-v • 10:05 min • September 30th, 2018
September 30th, 2018 •
*1,2, *1,2, *1, *1,3, *1,3, 1
1College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, 2Academy for Advanced Interdisciplinary Studies, Peking University, 3PKU-Tsinghua-NIBS Graduate Program, Peking University
* These authors contributed equally

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Vi beskriver en metode til isolering af endokrine celler fra embryonale, neonatal og postnatal bugspytkirtel efterfulgt af encellede RNA sekventering. Denne metode giver mulighed for analyser af pancreas endokrine lineage udvikling, celle heterogenitet og transkriptom dynamik.

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Udviklingsmæssige biologi sag 139 bugspytkirtlen endokrine celler β celle encellede RNA-sekventering Gen-ekspression cellulære heterogenitet
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