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İzotop seyreltme HPLC-MS/MS kritik hastalıklar içinde antibiyotik ile tedavi edici ilaç izleme multiplex
JoVE Journal
Medicine
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JoVE Journal Medicine
Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses
DOI:

11:17 min

August 30, 2018

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Chapters

  • 00:04Title
  • 01:05Preparation of the Calibrators, Quality Control Samples, and Internal Standards Mix
  • 02:29Buffer Preparation for Chromatography
  • 03:47Sample Cleanup and HPLC-MS/MS Analysis
  • 06:28Quality Assessment and Quantification
  • 08:14Results: Data Analysis and Quantification of Piperacillin
  • 09:44Conclusion

Summary

Automatic Translation

Burada bir tandem kütle spektrometresi tabanlı protokol sık kullanılan antibiyotikler miktar yoğun bakım üniteleri, yani cefepime, meropenem, Siprofloksasin, moxifloxacin, linezolid ve piperacillin mevcut.

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