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同位素稀释-高效液相色谱-抗生素在危重症患者中的多重治疗药物监测

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JoVE Journal Medicine

Multiplex Therapeutic Drug Monitoring by Isotope-dilution HPLC-MS/MS of Antibiotics in Critical Illnesses

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同位素稀释-高效液相色谱-抗生素在危重症患者中的多重治疗药物监测

DOI:

10.3791/58148-v

•

11:17 min

•

August 30, 2018

•

Carina Schuster, Sebastian Sterz, Daniel Teupser, Mathias Brügel, Michael Vogeser, Michael Paal

¹Institute of Laboratory Medicine, University Hospital,LMU Munich

Chapters

00:04Title
01:05Preparation of the Calibrators, Quality Control Samples, and Internal Standards Mix
02:29Buffer Preparation for Chromatography
03:47Sample Cleanup and HPLC-MS/MS Analysis
06:28Quality Assessment and Quantification
08:14Results: Data Analysis and Quantification of Piperacillin
09:44Conclusion

Summary

Automatic Translation

在此, 我们提出了一种基于串联质谱的协议, 用于定量治疗重症监护病房中的常用抗生素, 即头孢吡肟、美罗培南、环丙沙星、莫西沙星、利奈和哌拉西林。

Tags

Keywords: Antibiotics Therapeutic Drug Monitoring Mass Spectrometry HPLC-MS/MS Intensive Care Cefepime Meropenem Ciprofloxacin Moxifloxacin Linezolid Piperacillin Pharmacokinetics Serum Calibrators Quality Controls Internal Standards Mobile Phase Formic Acid Ammonium Formate

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