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JoVE Journal
Bioengineering

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JoVE Journal Bioengineering
Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells
 

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells

Article DOI: 10.3791/58231-v • 08:15 min • September 28th, 2018
September 28th, 2018 •
1, 1, 1, 1, 1, 1, 1, 1, 1
1Center for Drug Evaluation and Research, Office of Product Quality, Office of Biotechnology Products, Division of Biotechnology Review and Research II, U.S. Food and Drug Administration

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요약
Automatic Translation

A detailed protocol for the concurrent operation of 48 parallel cell cultures under varied conditions in a microbioreactor system is presented. Cell culture process, harvest and subsequent antibody titer analysis are described.

Tags

High-Throughput Automated Microbioreactor System Production Model IgG1 CHO Cells Cell Line Characterization Process Optimization Shake Flasks Automation Small Scale Studies Data Generation Sai Rashmika Velugula Casey Kohnhorst Research Fellows Freezing Medium Rapidly Thawed Cell Suspension OptiCHO Medium Cell Culture Incubator Subculturing Spinner Flask Viability
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