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Immunology and Infection

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JoVE Journal Immunology and Infection
Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies
 

Humanized NOD/SCID/IL2rγnull (hu-NSG) Mouse Model for HIV Replication and Latency Studies

Article DOI: 10.3791/58255-v • 07:10 min • January 7th, 2019
January 7th, 2019 •
1, 1, 1,2, 1, 1
1Department of Molecular and Cellular Biology, Beckman Research Institute of City of Hope, 2Irell and Manela Graduate School of Biological Sciences, Beckman Research Institute of City of Hope

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요약
Automatic Translation

This protocol provides a method to establish humanized mice (hu-NSG) via intrahepatic injection of human hematopoietic stem cells into radiation-conditioned neonatal NSG mice. The hu-NSG mouse is susceptible to HIV infection and combinatorial antiretroviral therapy (cART) and serves as a suitable pathophysiological model for HIV replication and latency investigations.

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Keywords: Hu-NSG Mouse Model HIV Replication HIV Latency Non-invasive Human Stem Cells CD34+ HSCs NOD/SCID/IL2rγnull Mice CART HIV Infection Engraftment Validation Flow Cytometry
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