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Immunology and Infection

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JoVE Journal Immunology and Infection
Antigene liposomen voor generatie van ziekte-specifieke antilichamen
 
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Antigene liposomen voor generatie van ziekte-specifieke antilichamen

Article DOI: 10.3791/58285-v • 10:31 min • October 25th, 2018
October 25th, 2018 •
*1, *2,3, *3,4, 5, 6, 3,4, 5,7
1Janssen R&D, 2Department of Microbiology and Immunology, University of North Carolina, 3UNC Food Allergy Initiative, University of North Carolina, 4Department of Pediatrics, University of North Carolina, 5Department of Chemistry, University of Alberta, 6Department of Molecular Medicine, Scripps Research Institute, 7Department of Medical Microbiology and Immunology, University of Alberta
* These authors contributed equally

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Beschreven is de voorbereiding van de antigene Liposomale nanodeeltjes en hun gebruik in stimulerende B-cel activatie in vitro en in vivo. Consistente en robuuste antilichaam reacties geleid tot de ontwikkeling van een nieuw model van de pindaallergie. Het protocol voor het genereren van antigene liposomen kan worden uitgebreid tot verschillende antigenen en immunisatie modellen.

Tags

Immunologie en infecties probleem 140 nanodeeltjes liposomen anafylaxie allergie van de pinda Calcium Flux Stroom Cytometry specifieke antigeen Ara h 2 voedselallergie IgE
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