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全猪胰腺的分离与去细胞

JoVE 신문
생체공학

This content is Free Access.

JoVE 신문 생체공학

Isolation and Decellularization of a Whole Porcine Pancreas

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全猪胰腺的分离与去细胞

DOI:

10.3791/58302-v

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09:08 min

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October 10, 2018

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Vijay Kumar Kuna*¹, Niclas Kvarnström*², Erik Elebring, Suchitra Sumitran Holgersson

¹Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy,University of Gothenburg, ²The Transplant Institute,Sahlgrenska University Hospital

Chapters

00:04Title
01:01Porcine Pancreas Dissection
04:51Decellularization Preparation
06:00Porcine Pancreas Decellularization
07:33Results: Representative Gross Morphology and H&E Staining of Normal and Decellularized Pancreata
08:11Conclusion

Summary

자동 번역

整个胰腺组织工程是一个挑战, 因为它的外分泌和内分泌功能。我们展示了一个完整的猪胰的解剖方法和成功的去细胞的过程中, 清洁剂海卫 X-100, 钠脱氧和脱氧核糖核酸酶的灌注。

Tags

Keyword Extraction: Porcine Pancreas Decellularization Dissection Blood Vessels Peristaltic Pump Organ Chamber Detergent Container Detergent Collection Container Heparinized Female Pig Midline Incision Splenic Lobe Duodenal Lobe Connection Lobe Major Duodenal Papilla Inferior Esophagus Small Intestine Colon

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