JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Priority Research Centre for Reproductive Science, School of Environmental and Life Sciences,University of Newcastle, 2Hunter Medical Research Institute, 3Department of Physiology, Turku Center for Disease Modeling, Institute of Biomedicine,University of Turku, 4School of Biomedical Sciences and Pharmacy, Faculty of Health and Medicine,University of Newcastle
Chapters
- 00:04Title
- 00:36Immunofluorescence Staining of the Paraffin-embedded Epididymal Sections
- 02:44Isolation of Epididymosomes from the Mouse Caput Epididymis
- 05:26Immunofluorescence Staining of mECap18 Cells
- 06:22Isolation of Proteins from Conditioned Cell Culture Medium
- 08:06Results: Analysis of Epididymal Protein Synthesis and Secretion
- 09:45Conclusion
Here, we report the immunofluorescence localization of dynamin to illustrate the protocols for the detection of proteins in paraffin-embedded mouse epididymal sections and those of an immortalized epididymal cell line (mECap18). We also describe the protocols for the isolation of secretory proteins from both epididymal fluid and conditioned cell media.
