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体内新生小鼠皮层神经元的双光子成像

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In Vivo Two-photon Imaging of Cortical Neurons in Neonatal Mice

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体内新生小鼠皮层神经元的双光子成像

DOI:

10.3791/58340-v

•

06:24 min

•

October 18, 2018

•

Hidenobu Mizuno^2,3, Shingo Nakazawa³, Takuji Iwasato³

¹International Research Center for Medical Sciences (IRCMS),Kumamoto University, ²Division of Neurogenetics,National Institute of Genetics, ³Department of Genetics,SOKENDAI (The Graduate University for Advanced Studies)

Chapters

00:04Title
00:33Exposing the Skull
01:29Cranial Window Preparation
03:29Two-Photon Imaging
04:59Results: In vivo Two-Photon Imaging of Cortical Neurons in Neonatal Mice
05:57Conclusion

Summary

자동 번역

我们提出了一种用于成像新生小鼠大脑皮层的活体双光子成像协议。该方法适用于分析皮层神经元的发育动态、控制神经元动力学的分子机制以及疾病模型神经元动力学的变化。

Tags

Keywords: In Vivo Two-photon Imaging Cortical Neurons Neonatal Mice 신경과학 Neuron Morphology Cranial Window Anesthesia Craniotomy Agarose Gel Cover Slip Dental Cement Titanium Bar Analgesic

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