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Biochemistry

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JoVE Journal Biochemistry
Studium der Protein Import in Chloroplasten mit Protoplasten
 
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Studium der Protein Import in Chloroplasten mit Protoplasten

Article DOI: 10.3791/58441-v • 06:29 min • December 10th, 2018
December 10th, 2018 •
1, 1, 1,2
1Division of Integrative Biosciences and Biotechnology, Pohang University of Science and Technology, 2Department of Life Sciences, Pohang University of Science and Technology

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Hier beschreiben wir ein Protokoll, um Proteine in Protoplasten zum Ausdruck zu bringen, mit PEG-vermittelten Transformationsmethode. Die Methode bietet einfach Ausdruck der interessierenden Proteine und effiziente Untersuchung von Protein-Lokalisierung und der Importvorgang für verschiedenen experimentellen Bedingungen in-vivo.

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Biochemie Ausgabe 142 Protoplasten Chloroplasten Arabidopsis Protein Import Polyethylenglykol transiente Expression
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