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Journal / Neuroscience / 定量自动放射法测定维沃脑蛋白合成区域速率…
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JoVE Journal Neuroscience
定量自动放射法测定维沃脑蛋白合成区域速率
 
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定量自动放射法测定维沃脑蛋白合成区域速率

Article DOI: 10.3791/58503-v • 11:01 min • June 28th, 2019
June 28th, 2019 •
1, 1, 1, 1, 1
1Section on Neuroadaptation and Protein Metabolism, National Institute of Mental Health, National Institutes of Health

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요약
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蛋白质合成是细胞的关键生物过程。在大脑中,它是自适应变化所必需的。测量完整大脑中蛋白质合成率需要仔细的方法学考虑。这里我们介绍了L-[1-14C]-亮细胞定量自射方法,用于测定体内脑蛋白合成的区域速率。

Tags

神经科学 问题 148 蛋白质合成 大脑 蛋白质降解 自光,翻译 氨基酸 非糖霉素
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