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1MCN Junior Research Group, Munich Center for Neurosciences,Ludwig Maximilian Universitat, BioMedical Center, 2Institute of Stem Cell Research, Helmholtz Zentrum,German Research Center for Environmental Health, 3Institute of Developmental Genetics, Helmholtz Zentrum,German Research Center for Environmental Health, 4Physiological Genomics,Ludwig Maximilian Universitat, BioMedical Center
Chapters
- 00:04Title
- 00:34Design of STAgR Cloning Primers with Overhangs
- 01:22Generation of STAgR Cloning Fragments
- 03:56Gibson Assembly Reaction and Bacterial Transformation
- 05:50Selecting STAgR Clones by Bacterial Colony PCR
- 08:08Results: Analysis of String Assembly gRNA Cloning
- 09:27Conclusion
