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优化干扰素伽隆弹性体检测在几内亚猪模型接种疫苗后 t 细胞反应

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Optimized Interferon-gamma ELISpot Assay to Measure T Cell Responses in the Guinea Pig Model after Vaccination

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优化干扰素伽隆弹性体检测在几内亚猪模型接种疫苗后 t 细胞反应

DOI:

10.3791/58595-v

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08:13 min

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January 20, 2019

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Katherine Schultheis, Hubert Schaefer, Holly M. Pugh, Bryan S. Yung, Janet Oh, Jacklyn Nguyen, Laurent Humeau, Kate E. Broderick, Trevor R.F. Smith

¹Inovio Pharmaceuticals, Inc, ²Mycotic and Parasitic Agents and Mycobacteria,Robert Koch Institute

Chapters

00:04Title
00:58Preparation of ELISpot-plates and Cell-stimulation
02:09Blood Sample Processing
03:44Plate Preparation and Cell-plating
04:47Detection of Interferon-gamma Positive Spots
06:34Results: ELISpot Assay Used to Characterize T-cell Responses in Guinea Pigs
07:15Conclusion

Summary

자동 번역

对豚鼠 pbmc 进行干扰素-γ elispot 检测的发展, 使其能够在这一高度相关的传染病研究模型中描述 t 细胞的反应。我们已经应用该检测方法来测量 t 细胞与 dna 疫苗的皮内传递相关的反应。

Tags

Keywords: ELISpot Assay T Cell Responses Guinea Pig Model Vaccination Immunotherapeutics Humoral Immunity Peripheral Blood Kinetics Capture Anti-guinea Pig Interferon Gamma Antibody VE4 Density Gradient Medium PBMCs

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