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JoVE Journal Chemistry
Grootschalige topdown Proteomics met behulp van capillaire Zone elektroforese Tandem massaspectrometrie
 
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Grootschalige topdown Proteomics met behulp van capillaire Zone elektroforese Tandem massaspectrometrie

Article DOI: 10.3791/58644-v • 10:05 min • October 24th, 2018
October 24th, 2018 •
*1, *1, 1, 2, 2,3, 1
1Department of Chemistry, Michigan State University, 2Department of BioHealth Informatics, Indiana University-Purdue University Indianapolis, 3Center for Computational Biology and Bioinformatics, Indiana University School of Medicine
* These authors contributed equally

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Een gedetailleerd protocol wordt beschreven voor de scheiding, identificatie en karakterisering van proteoforms in EiwitSteekproeven met behulp van capillaire zone elektroforese-electrospray ionisatie-tandem massaspectrometrie (CZE-ESI-MS/MS). Het protocol kan worden gebruikt voor de hoge-resolutie karakterisatie van proteoforms in simple EiwitSteekproeven en de grootschalige identificatie van proteoforms in complexe Proteoom monsters.

Tags

Chemie kwestie 140 Top-down proteomics capillaire zone elektroforese electrospray ionisatie Spectrometrie van de massa van het tandem proteoform Escherichia coli
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