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Immunology and Infection

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JoVE Journal Immunology and Infection
Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing
 

Identification of Mouse and Human Antibody Repertoires by Next-Generation Sequencing

Article DOI: 10.3791/58804-v • 08:51 min • March 15th, 2019
March 15th, 2019 •
1, 2, 3, 1, 4,5, 4, 4, 6, 7,8, 4,5, 9, 2, 9,10
1Graduate School of Life and Environmental Sciences, University of Tsukuba, 2Center for Influenza Virus Research, National Institute of Infectious Diseases, 3School of Science and Technology, Kwansei Gakuin University, 4Department of Pathology, National Institute of Infectious Diseases, 5Division of Infectious Diseases Pathology, Department of Global Infectious Diseases, Tohoku University Graduate School of Medicine, 6Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University, 7Division of Collaboration and Education, Research Center for Zoonosis Control, Hokkaido University, 8Global Station for Zoonosis Control, GI-CoRE, Hokkaido University, 9Department of Immunology, National Institute of Infectious Diseases, 10Faculty of Life and Environmental Sciences, University of Tsukuba

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Here, we describe protocols for the analysis and visualization of the structure and constitution of whole antibody repertoires. This involves the acquisition of vast sequences of antibody RNA using next-generation sequencing.

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Keywords: Antibody Repertoire Next-generation Sequencing B Cell Repertoire Immune Response Vaccine Efficacy Immunoglobulin Amplification RNA Extraction Mouse Tissue
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