Waiting
로그인 처리 중...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Cancer Research

이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.

Preparation of Exosomes for siRNA Delivery to Cancer Cells
 

Preparation of Exosomes for siRNA Delivery to Cancer Cells

Article DOI: 10.3791/58814-v 09:59 min December 5th, 2018
December 5th, 2018

챕터

요약

An exosome is a new generation of drug delivery carriers. We established an exosome isolation protocol with high yield and purity for siRNA delivery. We also encapsulated fluorescently labelled non-specific siRNA into exosomes and investigated the cellular uptake of siRNA-loaded exosomes in cancer cells.

Tags

Keywords: Exosomes SiRNA Delivery Cancer Cells Bioreactor Ultracentrifugation Sucrose Cushion HEK-293 Cells Exosome-depleted Medium Pre-clearing Centrifugation
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter