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放射性脉冲追逐对活细胞蛋白质折叠、迁移和降解的分析

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Analysis of Protein Folding, Transport, and Degradation in Living Cells by Radioactive Pulse Chase

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放射性脉冲追逐对活细胞蛋白质折叠、迁移和降解的分析

DOI:

10.3791/58952-v

•

08:59 min

•

February 12, 2019

•

Nicholas McCaul², Hui Ying Yeoh, Guus van Zadelhoff, Naomi Lodder, Bertrand Kleizen, Ineke Braakman

¹Cellular Protein Chemistry,Utrecht University, ²Program in Cellular and Molecular Medicine,Boston Children’s Hospital

Chapters

00:04Title
01:05Pulse Chase for Adherent Cells
04:07Pulse Chase for Suspension Cells
05:03Immunoprecipitation
06:16Analysis of the Radioactive Pulse Chase Results
07:58Conclusion

Summary

자동 번역

在这里, 我们描述了一种一般脉冲追逐方法的协议, 该方法允许在活细胞中对蛋白质的折叠、迁移和降解进行动力学分析。

Tags

Keywords: Protein Folding Protein Transport Protein Degradation Radioactive Pulse Chase 35S-labeled Methionine And Cysteine Protein Biosynthesis Co- And Post-translational Modifications Disulfide Bond Formation Acetylation Cell Culture Transfection Starvation Medium Pulse Solution Chase Medium Stop Buffer

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