JoVE Journal
Bioengineering
Bioengineering
이 콘텐츠 오픈 액세스로 구독없이 시청하실 수 있습니다.
챕터
요약
Long-read sequences greatly facilitate the assembly of complex genomes and characterization of structural variation. We describe a method to generate ultra-long sequences by nanopore-based sequencing platforms. The approach adopts an optimized DNA extraction followed by modified library preparations to generate hundreds of kilobase reads with moderate coverage from human cells.