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Immunology and Infection

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JoVE Journal Immunology and Infection
Discriminatie van zeven immuun cel deelverzamelingen door twee-fluorescerende Stroom Cytometry
 
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Discriminatie van zeven immuun cel deelverzamelingen door twee-fluorescerende Stroom Cytometry

Article DOI: 10.3791/58955-v • 10:58 min • March 5th, 2019
March 5th, 2019 •
1, 2
1Oncology Research, Medimmune, LLC, 2Division of Rheumatology, Johns Hopkins University School of Medicine

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Hier presenteren we een stroom cytometrische protocol ter identificatie van CD4+ en CD8+ T cellen en γδ T cellen, B-cellen, NK-cellen, monocyten in perifere bloed met behulp van slechts twee fluorochromes in plaats van zeven. Met deze aanpak, kunnen vijf extra markeringen op de meeste flow cytometers worden opgeslagen.

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Immunologie en infecties probleem 145 stroom cytometry immunologie immunophenotyping perifere bloed fluorescerende geduldig multiparametric analyse.
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