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فحص فاعليه في الوقت الحقيقي للخلايا التائية لمستقبلات مستضد تشياريك التي تستهدف الخلايا السرطانية الصلبة والدموية
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Chapters
- 00:04Title
- 00:30Chimeric Antigen Receptor (CAR)-Encoding Lentivirus Generation
- 02:29CAR T Cell Generation and Expansion
- 03:16CAR Expression Detection
- 04:55Real-Time Cytolysis Potency Assay
- 06:48Results: Representative CAR T Cell Target Cell Killing Efficacy as Assessed by RTCA
- 08:05Conclusion
Tags
Real-time Potency AssayChimeric Antigen Receptor T CellsSolid And Hematological Cancer CellsCELLigence Real-time Cell AnalysisLabel-free MonitoringTarget Cancer Cell KillingEffector Cell TypesCombination TherapiesLentiviral VectorHEK293FT CellsTransfectionLentivirus ProductionUltracentrifugationQuantitative RT-PCRCAR-T Cell GenerationPeripheral Blood Mononuclear CellsCD3/CD28 Coated Microbeads
