JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
1Department of Chemistry,Brooklyn College, The City University of New York, 2Ph.D. Program in Chemistry,The Graduate Center of The City University of New York, 3Ph.D. Program in Biochemistry,The Graduate Center of The City University of New York, 4Department of Genetics and Genomic Sciences,Icahn School of Medicine at Mount Sinai, 5Women’s Health Research Institute,Icahn School of Medicine at Mount Sinai, 6Laboratory for Translational Research,Western Connecticut Health Network
Chapters
- 00:04Title
- 00:35Synthesis of Oleic Acid-Platinum(II) Conjugate
- 02:41Preparation of Unmodified and Fluorescein Isothiocyanate (FITC)-Modified Lysine-Tyrosine-Phenylalanine (KYF) Tripeptide
- 05:59Synthesis of FITC-KYF-Stabilized or KYF-Stabilized Nanoemulsion with Oleic Acid-Pt(II) Cores (FITC-KYF-Pt-NE or KYF-Pt-NE)
- 08:03Results: KYF-Pt-NE and FITC-KYF-Pt-NE Characterization
- 09:43Conclusion
Questo protocollo descrive un metodo efficiente per sintetizzare una Nanoemulsione di un coniugato acido oleico acids-platinum(II) stabilizzato con un tripeptide di lisina-tirosina-fenilalanina (KYF). Le forme di Nanoemulsione in condizioni blande sintetiche tramite auto-assemblaggio del KYF e il coniugato.
